Helps correct for technical variability within FlowJo by normalizing batches of flow data. The Workspace contains a list of all of the data samples that you load, the gates, statistics and other analyses that you apply, and the table and graphical layout that you design Automatically Find the Shortest Gating Strategy to a Population of Interest. This is called a Gating Tree FlowJo is an integrated environment for viewing and analysing flow cytometric data, presented in the form of a Workspace. When you create gates in a sample or subpopulation, FlowJo shows the new subsets in the Workspace in a hierarchical manner each subpopulation is shown indented under the sample or subset that it came from. The backgating icon shows up graph window for a subset that is gated more than once (or you can choose Backgating Analysis. FlowJo's backgating analysis provides a tool to view the effect of every gate in the gating tree on the final gated population. 2) Take (gate) 3 and apply it to (gate) 1, creating a new subpopulation we'll call 3b. You tell FlowJo to backgatehere is what FlowJo does: 1) Take (gate) 3 and apply it to (gate) 2 (you'll see no change in the workspace). Isolate, divide, or group your populations for excellent annotation and analysis FlowJo vX has expanded gating tool options to help you effectively gate your data.This menu allows you to choose whether to. Show Child graph: This allows you to select what parameters FlowJo will use on the axes when you open the graph of a sub-population. Use Gating-ML conventions: Check this box to save a second file besides the workspace file that describes the gates in the workspace using this industry standard format.When you set a gate to select a subset of the cells, FlowJo creates a new node-i.e., a new population When you collect a sample and import it into the workspace, FlowJo creates a node to represent that set of events. The process of gating simply creates a new population.
FlowJo offers a whole set of Gating and Advanced Gating tools
Subsets can be gated to generate further subsets, until a collection has only the cells for which a graphic display or analysed statistics are desired. Gating : Subsetting collected events for further analysis is critical in analysis of flow cytometry data.Home FlowJo gating Graphs and Gating FlowJo Documentation - Just another